硫酸葡聚糖 钠盐 来源于明串珠菌 属

Dextran sulfate sodium (DSS) is a synthetic sulfated derivative of dextran, a complex polysaccharide composed of glucose molecules. DSS is characterized as a biocompatible, highly branched polysaccharide with 1–6 and 1–4 glycosidic linkages, featuring approximately 2.3 sulfate groups per glucosyl unit. Dextran sulfate is a sulfated polysaccharide with a variable molecular weight ranging from 5 to 1,400 kDa. It finds extensive use in molecular biology and biochemical research, with a particular emphasis on gastrointestinal investigations.DSS is widely recognized for its ability to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD). It is widely recognized for its capacity to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD) by creating models of acute, chronic, and relapsing IBD, by adjusting its concentration and administration frequency. Furthermore, Dextran sulfate is applied in various molecular biology techniques, such as precipitating lipoproteins, accelerating DNA probe hybridization, and facilitating ribosomal isolations. Notably, DSS serves as a valuable cross-linker for chitosan, allowing for the creation of microparticles and nanoparticles used in controlled drug delivery systems. Beyond these applications, DSS plays a central role in diverse research areas, particularly immunology and virology, where it actively modulates immune responses, enhances humoral immunity, influences B-lymphocyte activation, and affects thymocyte reactivity to lectins.

葡聚糖硫酸酯具有许多用途，当进行核酸杂交时可用作缩短探针退火所需时间的促进剂。当杂交速率成为检测稀有序列的限制因子时或当可用探针数量有限时，可在杂交缓冲液中加入葡聚糖硫酸酯。葡聚糖硫酸酯通过从聚合物所占溶液体积中扣除核酸而发挥促进剂的作用，从而提高有效的探针浓度。

Dextran sulfate has been used

• as a molecular weight marker to assess the purity and homogeneity of the purified polysaccharides by gel electrophoresis[1]
• as a component of the hybridization buffer in fluorescence in situ hybridization (FISH) protocol for DNA detection and analysis[2] [3]

Dextran sulfate, being a sulfated polysaccharide, consistently triggers experimental acute and chronic colitis. It is believed to initiate mucosal injury and inflammation, primarily through a direct toxic impact on epithelial cells. This initial effect activates macrophages and T lymphocytes, ultimately leading to cytokine-mediated cytotoxicity.

• Suitable for Nucleic acid hybridization & Molecular Biology
• Dextran Sulfate with an average molecular weight of >500,000
• Sulfur Content of 16.0-19.0 %
• Versatile and adaptable for wide variety of laboratory and research applications

已测试了在核酸杂交中的适应性

This product is intended for research purposes only, and it is not meant for human consumption.